CHOLINESTERASE AND TYROSINASE ENZYME INHIBITORY EFFECTS AND POSSIBLE NEUROPROTECTIVE POTENTIAL OF ACCORN FRUIT AND COFFEES HARVESTED FROM QUERCUS COCCIFERA
Fatma Sezer Şenol1, Nazım Şekeroğlu2, Sevgi Gezici3, Esra Kılıç4, İlkay Erdoğan Orhan1
1 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, Turkey, firstname.lastname@example.org
2 Department of Medicinal and Aromatic Plants, Kilis 7 Aralık University, Kilis, Turkey
3 Department of Biology, Molecular Biology & Genetic, Faculty of Art and Science, University of Gaziantep, Gaziantep, Turkey,
4 Department of Biology, Kilis 7 Aralık University, Kilis, Turkey
Objective / Purpose: Neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD) are among the deadly disorders affecting elderly population. Unfortunately, effective treatments and medications are still absent to seize the disease. The ethanol extracts of the raw peeled acorns and acorn coffees prepared by two different methods (boiled-roasted ground and roasted ground) were tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR) the enzymes connected to neurodegeneration.
Material and Methods: Acorns used in the present study were harvested from wild Quercus coccifera L. tree and shrubs in Kilis province, located in the Eastern Mediterranean Region in November 2016. Cholinesterase and TYR inhibitory activity of the ethanol extracts prepared from raw material and two coffee samples were screened using ELISA microtiter assay at 2 mg/mL stock concentration. Antioxidant effect of the extracts was also tested for their scavenging activity against DPPH, while total phenol and flavonoid quantities of the extracts were determined spectrophotometrically.
Results: Our results indicated that all of the extracts had a notable AChE and BChE inhibition, while they were inactive against TYR. The most active extract against cholinesterases was prepared from raw material which had 65.94 ± 3.59 % and 85.04 ± 3.83 % inhibition against AChE and BChE, respectively.
Conclusion / Discussion: Our data indicated that the fruit extract from Quercus coccifera emerged as the sources of possible cholinesterase inhibitors and deserves future studies.
Keywords: Acetylcholinesterase, butyrylcholinesterase, enzyme inhibition, Quercus coccifera
 Sekeroglu, N., Ozkutlu, F., & Kilic, E. (2017). Mineral composition of acorn coffees. Indian Journal of Pharmaceutical Education and Research, 51(3), 136-139.